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91.
Accelerated belowground C cycling in a managed agriforest ecosystem exposed to elevated carbon dioxide concentrations 总被引:2,自引:0,他引:2
We investigated the effects of three elevated atmospheric CO2 levels on a Populus deltoides plantation at Biosphere 2 Laboratory in Oracle Arizona. Stable isotopes of carbon have been used as tracers to separate the carbon present before the CO2 treatments started (old C), from that fixed after CO2 treatments began (new C). Tree growth at elevated [CO2] increased inputs to soil organic matter (SOM) by increasing the production of fine roots and accelerating the rate of root C turnover. However, soil carbon content decreased as [CO2] in the atmosphere increased and inputs of new C were not found in SOM. Consequently, the rates of soil respiration increased by 141% and 176% in the 800 and 1200 μL L?1 plantations, respectively, when compared with ambient [CO2] after 4 years of exposure. However, the increase in decomposition of old SOM (i.e. already present when CO2 treatments began) accounted for 72% and 69% of the increase in soil respiration seen under elevated [CO2]. This resulted in a net loss of soil C at a rate that was between 10 and 20 times faster at elevated [CO2] than at ambient conditions. The inability to retain new and old C in the soil may stem from the lack of stabilization of SOM, allowing for its rapid decomposition by soil heterotrophs. 相似文献
92.
McIntyre C. L. Whan V. A. Croft B. Magarey R. Smith G. R. 《Molecular breeding : new strategies in plant improvement》2005,16(2):151-161
Marker-assisted selection for traits that are difficult to screen for, such as resistance to many sugarcane diseases, has
the potential to facilitate the development of improved cultivars in sugarcane. Pachymetra root rot (PRR) and brown rust resistance
ratings were obtained over two years for 192 I1 progeny (progeny produced by two heterozygous, non-inbred parental lines)
of a sugarcane (Saccharum spp. hybrid) cross between two elite sugarcane clones, Q117 and 74C42. Approximately 1000 single-dose markers, including
microsatellite (SSR), amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) markers,
were scored across the population and maps containing approximately 400 markers were constructed for each parent. At p ≤ 0.01, two genomic regions, one from the female Q117 map and a different region from the 74C42 male map, plus an unlinked
bi-parental simplex marker (single-dose marker present in both parents) were identified as associated with PRR over both years
of data collection. These regions explained between 6 and 16% of the phenotypic variation. An additional region was identified
in the female map as associated with PRR at p ≤ 0.01 in one year and p ≤ 0.05 in the second year. This region explained between 4 and 8% of the phenotypic variation. For brown rust, two genomic
regions, one from the female map and one from the male map, plus an unlinked marker from both maps, were identified as associated
with brown rust resistance at p ≤ 0.01 over two years of phenotypic data. Each region explained between 7 and 18% of the phenotypic variation. Several additional
regions were identified in both maps as associated with brown rust at p ≤ 0.01 in one year and p ≤ 0.05 in the second year. These regions also explained between 5 and 11% of the phenotypic variation. To validate these
markers and determine whether they would be useful in alternative germplasm, markers from each genomic region associated with
PRR or brown rust were screened across a set of 154 elite sugarcane clones; PRR and brown rust ratings were available for
131 and 72 of the clones, respectively. For PRR, three of the 6 markers tested remained significantly associated (p ≤ 0.01) with resistance ratings in the elite clone set. For brown rust, only one of the seven markers tested remained significantly
associated (p ≤ 0.01) with resistance in the elite clone set, with one other marker associated at p ≤ 0.05. These results suggest that these markers could be broadly effective in selecting for PRR and/or brown rust resistance
in sugarcane breeding programs. 相似文献
93.
Chun-Xiang Fu De-Xiu Zhao Xiao-Feng Xue Zhi-Ping Jin Feng Shan Ma 《Process Biochemistry》2005,40(12):3789-3794
Agrobacterium rhizogenes-mediated genetic transformation of Saussurea involucrata was investigated. Four bacterial strains, A4, LBA 9402, R1000 and R1601 and three explant types, leaf blade, petiole and root, were examined. Over 100 hairy root lines were successfully established with strains R1601, R1000 and LBA9402, but none with A4. The highest transformation efficiency of 67% was achieved by using strain R1601 with root explants. One hairy root line isolated from this combination, HR1601-1, produced up to 43.5 ± 1.13 mg syringin g−1 dw, which is about 50-fold higher than that in the wild type plants.Two other lines, HR1000-1 and HRLBA9402-1, isolated from R1000- and LBA9402-transformed roots, respectively, also displayed high capacity of syringin production, being 32.5 ± 3.08 and 39.7 ± 1.37 mg syringin g−1 dw. These three lines were characterized in detail. Polymerase chain reaction analyses confirmed these root lines were of A. rhizogenes origin. 相似文献
94.
U.H. Jin J.A. Chun M.O. Han J.W. Lee Y.B. Yi S.W. Lee C.H. Chung 《Process Biochemistry》2005,40(12):3754-3762
Sesame (Sesamum indicum L.) hairy roots were transformed with a fungal (Aspergillus) phytase and their culture conditions were surveyed for the extra-cellular production of the recombinant phytase protein in shake flasks. Kanamycin resistance of sesame hairy roots was observed at 50 μg ml−1 kanamycin sulfate and southern hybridization analysis confirmed the existence of the phytase gene in the hairy root genomic DNA. The continuous dark condition was more effective for both the root growth and phytase production than light. Slightly higher root growth was determined at 30 °C than 26 °C in Murashige & Skoog (MS) medium supplemented with 3% sucrose, while the final phytase production was greatest in MS medium with 5 or 3% sucrose at both temperatures of 26 and at 30 °C. Among the culture media used, full-strength MS medium was exclusively efficient for production of the recombinant phytase. Most rapid increase rates in both the root growth and phytase production were detected at the 4th week of the culture periods and thereafter their rates began to decrease. Our results indicated that 5–6-week culture periods may be necessary for the maximal phytase production. Western analysis revealed that even though the phytase proteins expressed were measured with greater activities in the liquid medium than in the root tissues, they were still retained in the tissues. 相似文献
95.
《植物学报(英文版)》2005,47(3)
A better understanding of the mechanisms of water uptake by plant roots should be vital for improving drought resistance and water use efficiency (WUE). In the present study, we have demonstrated correlations between root system hydraulic conductivity and root characteristics during evolution using six wheat evolution genotypes (solution culture) with different ploidy chromosome sets (Triticum boeoticum Bioss., T. monococcum L.: 2n = 2x = 14; T. dicoccides Koern., T. dicoccon (Schrank) Schuebl.: 2n = 4x = 28;T. vulgare Vill., T. aestivum L. cv. Xiaoyan No. 6: 2n = 6x = 42). The experimental results showed that significant correlations were found between root system hydraulic conductivity and root characteristics of the materials with the increase in ploidy chromosomes (2x→6x) during wheat evolution. Hydraulic conductivity of the wheat root system at the whole-plant level was increased with chromosome ploidy during evolution, which was positively correlated with hydraulic conductivity of single roots, whole plant biomass,root average diameter, and root growth (length, area), whereas the root/shoot ratio had an inverse correlation with the hydraulic conductivity of root system with increasing chromosome ploidy during wheat evolution. Therefore, it is concluded that that the water uptake ability of wheat roots was strengthened from wild to modern cultivated species during evolution, which will provide scientific evidence for genetic breeding to improve the WUE of wheat by genetic engineering. 相似文献
96.
《植物学报(英文版)》2005,47(3)
Plants have evolved some mechanisms to maximize the efficiency of phosphorus acquisition.Changes in root architecture are one such mechanism. When Fraxinus mandshurica Rupr. seedlings were grown under conditions of low phosphorus availability, the length of cells in the meristem zone of the lateral roots was longer, but the length of cells in the elongation and mature zones of the lateral roots was shorter,compared with seedlings grown under conditions of high phosphorus availability. The elongation rates of primary roots increased as phosphorus availability increased, but the elongation rates of the branched zones of the primary roots decreased. The number of lateral root primordia and the length of the lateral roots decreased as phosphorus availability increased. The topological index (altitude slope) decreased as phosphorus availability increased, suggesting that root architecture tended to be herringbone-like when seedlings were grown under conditions of low phosphate availability. Herringbone-like root systems exploit nutrients more efficiently, but they have higher construction costs than root systems with a branching pattern. 相似文献
97.
Effects of Phosphorus Nutrient on the Hydraulic Conductivity of Sorghum (Sorghum vulgare Pers.) Seedling Roots Under Water Deficiency 总被引:1,自引:0,他引:1
Ting-WuLEI Qing-WuXUE 《植物学报(英文版)》2005,47(4):421-427
Hydroponic experiments were conducted in a growth chamber and changes in the hydraulic conductivity of sorghum (Sorghum vulgare Pers.) roots (Lpr) at the three-leaf stage were measured using the pressure chamber method. Water deficiency was imposed with polyethylene glycol (PEG) 6000 and the phosphorus (P) levels were controlled by complete Hoagland solution with and without P nutrient. The objective of this study was to investigate the effect of P nutrition on root Lpr under water deficiency. The results showed that the Lpr in P deficiency treatments decreased markedly, but the Lpr recovered to the same value as that of control when sufficient P was supplied for 4-24 h. Water deficiency decreased Lpr, but the hydraulic conductivity of the roots with sufficient P supply was still higher than that of plants without P supply. When resuming water supply, the Lpr of the water-deficient plants under P supply recovered faster than that of plants without P supply, which indicates that plants with sufficient P nutrient are more drought tolerant and have a greater ability to recover after drought. The treatment of HgCl2 indicated that P nutrient could regulate the Lpr by affecting the activity and the expression levels of aquaporins. 相似文献
98.
龚玉莲 《现代生物医学进展》2005,5(2):29-32
综述了何首乌的快速繁殖、愈伤组织的诱导和培养、毛状根的诱导和培养以及活性成分的产生,并展望了何首乌生物技术的前景。 相似文献
99.
Jaisoo Kim Yoon-Seok Chang M. Yavuz. Corapcioglu Malcolm C. Drew 《Plant and Soil》2005,269(1-2):213-224
The fate of 14C-2,4,6-trinitrotoluene ([U-14C]TNT) in soil/plant systems was studied using onion (Allium cepa L.) plants with only a single root. It was found that the single roots grew exponentially and that the rate of water uptake
of the onion plants increased exponentially, as well. The concentration of [U-14C] in the roots at first increased and then appeared to reach a steady state, while the [U-14C] concentration in the leaves was found to increase linearly with time. The [U-14C] concentration in the rhizosphere increased gradually, while in the bulk soil it decreased slowly. The accumulation of [U-14C] in the rhizosphere is likely to difference between movement into the rhizosphere (through advective mass flow of soil water
by root uptake) and its uptake into the roots. The distribution of 14C in the soil/plant system was found to be 60–85% in the soil solid phase, 7–11% in the soil liquid phase, <1% in the soil
air phase, <1% in the root compartment, and <0.01% in the leaf compartment. The maximum RCF (root concentration factor) value
for TNT and its derivates was found to be about 20, and the maximum TSCF (transpiration stream concentration factor) was 0.18.
These values can be changed by a variety of factors in soil-plant systems 相似文献
100.
Facilitative Root Interactions in Intercrops 总被引:3,自引:0,他引:3
Facilitation takes place when plants ameliorate the environment of their neighbours, and increase their growth and survival.
Facilitation occurs in natural ecosystems as well as in agroecosystems. We discuss examples of facilitative root interactions
in intercropped agroecosystems; including nitrogen transfer between legumes and non-leguminous plants, exploitation of the
soil via mycorrhizal fungi and soil-plant processes which alter the mobilisation of plant growth resources such as through
exudation of amino acids, extra-cellular enzymes, acidification, competition-induced modification of root architecture, exudation
of growth stimulating substances, and biofumigation. Facilitative root interactions are most likely to be of importance in
nutrient poor soils and in low-input agroecosystems due to critical interspecific competition for plant growth factors. However,
studies from more intensified cropping systems using chemical and mechanical inputs also show that facilitative interactions
definitely can be of significance. It is concluded that a better understanding of the mechanisms behind facilitative interactions
may allow us to benefit more from these phenomena in agriculture and environmental management. 相似文献